Illumina MiSeq run fail Question

Use this forum for general bioinformatics questions, or questions regarding genomics, proteomics, etc.

Moderators: mdfenko, Abhijeet Bakre, strom

Illumina MiSeq run fail Question

Postby gerberd » Nov 30 2016 5:12 am

Hi everyone, a few days ago we had a MiSeq run, but it failed for some reasons. My job was to find out what was the problem, but I had never been working with MiSeq run data before, so I need a little help. I have that first (and very strong) assumption, that the dispersion of the DNA solution on the flow cell was uneven (missing data in tiles, stdev of density was enormous, photos, etc), therefore the software could not work up the data properly. However I found out that in the Run Summary doc. the starting read no. is ~27 million, and the read no. PS is ~700k, but in the indexing QC doc. the starting read number is ~1,6 million, and the read no. PS is ~600k, and I wonder what does this means? Also I want to find out if this information supports my colleague's assumption, that the indexing of the DNA went wrong (in wetlab). Thanks in advance for help! :shock: :)
gerberd
newcomer
newcomer
 
Posts: 1
Joined: Nov 30 2016 5:07 am

Return to General Bioinformatics

Who is online

Users browsing this forum: No registered users and 1 guest

cron