Luminex coupling stability

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Luminex coupling stability

Postby Bead Coupler » Mar 16 2011 5:29 am

Hi couplers,

I am in my second year of my PhD thesis and started using Luminex half year ago. My LMX experiments are designed to genotype only few HLA subtypes and so far I have good results. I have designed a multiplex reaction and typed up to 400 samples and work well but now I am having troubles with a coupled beads stored at 4C for 5 months. According to Luminex guidelines oligonucelotides coupled beads are stored for up to 98 weeks in TE but I do not see this. I see a decrease of 50% of MFI.

Thanks in advance,

Bead Coupler
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