Reconstitution and dilution of cytokines

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.

Reconstitution and dilution of cytokines

Postby ScienCEtool » Aug 14 2017 8:58 am

Hi

Im am making growth media for single cell cultures.
I need to ad cytokines in very small concentrations and volumes.

I need to reconstitute 2 ug of a cytokine to 0.1-1.0 mg/mL.
Furthermore I need to dilute 0.1-1.0 mg/mL to a stock concentration of 10000 ng/mL

Can anybody help me on how to do this?


Kind regards,
ScienCEtool
newcomer
newcomer
 
Posts: 2
Joined: Aug 14 2017 8:46 am

Re: Reconstitution and dilution of cytokines

Postby mdfenko » Aug 15 2017 9:23 am

the magic formula for dilutions is:

c1v1=c2v2

2ug is .002mg, to prepare 1mg/ml...
talent does what it can
genius does what it must
i do what i get paid to do
mdfenko
ModSquad
ModSquad
 
Posts: 1456
Joined: Jan 24 2006 2:39 pm
Location: Staten Island, NY USA

Re: Reconstitution and dilution of cytokines

Postby ScienCEtool » Aug 15 2017 10:38 am

So to get a concentration of 0.1 mg/mL I would have to add 20 uL of water right?

To go from a concentration of 0.1 mg/mL to 10000 ng/mL:

0.1 mg = 0.1x10^-6

I would like a final volume of 50 uL

Using the formula:

0.1x10^6 ng/mL * V1 = 10000 ng/mL * 0.05 mL

V1 = 0.005 mL

Is that right?
ScienCEtool
newcomer
newcomer
 
Posts: 2
Joined: Aug 14 2017 8:46 am

Re: Reconstitution and dilution of cytokines

Postby mdfenko » Aug 15 2017 11:12 am

ScienCEtool wrote:So to get a concentration of 0.1 mg/mL I would have to add 20 uL of water right?

yes
To go from a concentration of 0.1 mg/mL to 10000 ng/mL:

0.1 mg = 0.1x10^-6

I would like a final volume of 50 uL

Using the formula:

0.1x10^6 ng/mL * V1 = 10000 ng/mL * 0.05 mL

V1 = 0.005 mL

Is that right?

10000ng/ml is 10ug/ml; 0.1mg/ml=100ug/ml; your stock is 10x; you got the right answer (5ul to make 50ul) but watch out for your units, you got some of them a little screwy.
talent does what it can
genius does what it must
i do what i get paid to do
mdfenko
ModSquad
ModSquad
 
Posts: 1456
Joined: Jan 24 2006 2:39 pm
Location: Staten Island, NY USA


Return to Cell Culture

Who is online

Users browsing this forum: No registered users and 1 guest