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RNAse treating live cells

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.
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RNAse treating live cells

Postby uncdoc » Aug 13 2012 2:55 pm

Is there a well established protocol to RNAse treat live cells (permeabalize and RNAse treat to get rid of the RNA, but not the protein)?

Thanks in advance!
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Re: RNAse treating live cells

Postby relaxin » Aug 15 2012 4:44 pm

You may try protein transfection reagent. A number of companies sell this reagent. Just follow the protocol that comes with the reagent.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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