I never do manual cell counting. I have been using MTT proliferation assay, but find out it cannot tell the difference of cell number between treatment and control, even though I can see the difference by eye-balling. That is why I am thinking of buying a cell counter. What is the price of ViCell? The TC10 has a list price of about $4K.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.