BioTechniques Molecular Biology Techniques Forums

Welcome to the BioTechniques Molecular Biology Forums, a science-based bulletin board for techniques, tips, and questions concerning molecular biology, cell biology, microscopy, and bioinformatics.

Skip to content

Assay difference

Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.
Post a reply

Assay difference

Postby Mitochondria » Mar 21 2012 5:54 am

hi
can anyone tell me what are main differences between cell proliferation assays and cytotoxicity assays ?
thanks
Mitochondria
newcomer
newcomer
 
Posts: 3
Joined: Dec 26 2011 4:22 am
Top

Re: Assay difference

Postby relaxin » Apr 19 2012 9:23 am

Cell proliferation assays measure the number of live cells and cytotoxicity assays measure the number of dead cells. However, cell proliferation assays, such as MTT or MTS assays, can also be used to measure the decrease of live cells, and hence the cytotoxic effect of the test substance. There are specific cytotoxicity assays, e.g. LDH-based cytotoxicity assay measures the amount of LDH leaked out from dead cells into the medium.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
relaxin
PI of Posters
PI of Posters
 
Posts: 6465
Joined: Jan 11 2006 12:40 pm
Location: Mauna Kea
Top
Post a reply

Return to Cell Culture

Who is online

Users browsing this forum: No registered users and 2 guests

Powered by phpBB © 2000, 2002, 2005, 2007 phpBB Group