I think I need to give you some more information. In this system, I am interested in observing the interplay between the proteins DAPK and Pin1 and how their interaction affects microtubule dynamics within an Alzheimer's disease model. My original idea was to have various mutant forms of Pin1 expressed where DAPK is either overexpressed or knocked down. To achieve this, I thought it would be possible to cross a transgenic hAPP mouse strain with a DAPK-/- or DAPK overexpressed strain and then transfect in mutants of Pin1 for my experiments. I only want the mutant form of Pin1 expressed so I would have to knockdown endogenous Pin1 expression with siRNA.
I looked up some information on this cell line. As I understand it, these cells would not naturally exhibit Alzheimer's disease characteristics however, I see a paper in which mutant APP was transfected into the cells to induce this phenotype. If I use this cell type, I would have to control the levels and amounts of all three proteins (hAPP, Pin1, and DAPK) through transfection. For DAPK and Pin1, I would also need to regulate endogenous expression levels with siRNA or some other system. I am assuming this would become complicated quickly and may not even work. Given this, is this cell line still the best choice?
Sorry, I am very new to all of this so thanks for your patience!