Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.
recently I'm working on the isolation of LECs from Lymph node. Unfortunately I found some problems after the processation of the Lymph node for the isolation of these cells: in particular I noticed that the fibroblasts, in their growth, are more noumerous than the other endothelial cells of interest. Moreover these fibroblasts tend to obstruct the minimal growth of the few BECs and LECs cells. Futhermore in the successive selection for endothelial cells with anti CD31 ABs with the magnetic coloumn, I don't succeed to extract endothelial cells, but only fibroblasts. So does exist any method in the processing way of the Lymph node to obtain a majority of endothelial cells? Or is there any method to prevent the fibroblast's growth?
Thank you. Have a nice way.
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- Joined: Apr 18 2012 5:46 am
- Location: Brescia, Italy
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