Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.
Has anyone had luck hydration dried white blood cells from a cotton substrate. I have been using low dilutions of tween 20 in PBS to float of and rehydrate the cells. I incubate the blood stain in this mix for 20 min, spin down the cells, smear/air dry the sample, then stain with H and E. I have been getting blue colored cell but they seem fractionated or dehydrated still.
- Posts: 1
- Joined: May 18 2012 11:13 am
Return to Cell Culture
Who is online
Users browsing this forum: No registered users and 2 guests