Freezing DMEM/B27/EGF/FGF media?

[brainsnomnom]

Hi all...my Google-fu is failing me on this one: I am a super busy clinician-researcher who needs to streamline things as much as possible time-wise since I'm forever trying to cram in a few minutes of lab time throughout the day in between millions of other things. I am doing some work with glioblastoma cell lines that grow in a serum-free media that is composed of DMEM with glucose and glutamine, plus B27 and EGF/bFGF. Can I pre-make large batches (500-ml or so), then aliquot out into single use conical tubes (ie: only one thaw per tube, wouldn't have repeat freeze-thaw cycles) and freeze in the -20 until I need them? This would make life much easier for me, if there would be no degradation of the components. I have seen some anecdotal stuff about freezing DMEM, but wonder if anyone can provide first hand knowledge of this being OK with this particular mix. Seems like if the individual components can be frozen then the mix should be OK, right? Thanks!

[relaxin]

I have no experience with freezing DMEM or other medium. I think the medium should be quite stable at 4 C. I would freeze the additives (B27, EGF and FGF) in small aliquots and add them to the medium before use.

[brainsnomnom]

Technical support at Cellgro says no problemo she used to do it all the time, just don't do multiple freeze-thaws. Person at ATCC gave me a "freezing of media not recommended" blah blah generic CYA statement. I have been using it and so far good results. I do looooooove me some streamlining of repetitive mind-numbing processes! Not to mention elimination of pipetting variables etc.

[relaxin]

Just to remember that when you thaw out the medium, invert the tube a few times to ensure everything is re-dissolved, and the medium is homogeneous before pipetting.