Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. CTL, cytokine release, B cell culture), cell lines/primary culture, reagents, etc.


Postby tanphu8291 » Nov 23 2015 7:21 pm

I would like to ask some basic questions regarding 3t3-l1 diff.

1) why are dexamethasone and IBMX added to differentiating media?
2) what should the confluence of the culture be before differentiating?
3) what would happen to subsequent differentiation if the stock culture reached 100% by the time of splitting?

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Re: 3t3-differentiation

Postby Joannajone » Jun 24 2016 4:18 am

IBMX is a competitive nonselective phosphodiesterase inhibitor which raises intracellular cAMP, activates PKA, inhibits cell proliferation and induces differentiation.

DEX up-regulates C/EBPα by the receptor, and regulates preadipocytes differentiation.

The effect of these reagents are Inhibiting the expression of gene which is related to proliferation, differentiation and promoting the expression of adipocyte-specific gene and finally differentiate into adipocytes.
Last edited by mdfenko on Jun 24 2016 6:28 am, edited 1 time in total.
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