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new to qpcr

Use this category for questions related to various quantitative PCR methods, including real time PCR, reverse transcription (RT)/PCR, qPCR, etc. For general PCR methods, continue to use the DNA/General PCR forum

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new to qpcr

Postby fathima » Jun 14 2012 5:14 am

Iam new to qPCR world.We have a miniopticon qPCR machine(BIORAD)
could any body post me a standard curve/graph produced by this machine and melting curve produced by this system.
because I used cDNA and amplified actin referance gene and targets gene.I had so many peakes in melting curve graph.
ALSO IS IT MUST TO HAVE SLOPE MORE -3.33? Y IT COMES AS -1.44?

PLEASE HELP IN ANSWERING
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Re: new to qpcr

Postby relaxin » Jun 20 2012 12:23 pm

I do not use a miniopticon qPCR machine. But, many peaks in melting graph mean nonspecific amplicons. You need to redesign your primers.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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