Use this category for questions related to various quantitative PCR methods, including real time PCR, reverse transcription (RT)/PCR, qPCR, etc. For general PCR methods, continue to use the DNA/General PCR forum
Moderators: r.rosati, Suzanne
The post docs in the lab are doing a lot of q-pcr from CHIP and want us to get a q-pcr machine for the lab. I was wondering which machines people are using and would they recommend them for ease of use, reliability etc?
- Posts: 21
- Joined: Nov 12 2003 9:44 am
we use a roche 480II
and we are quite happy about.
we have also a rotorgene from invitrogen...that we use for meting point variation study.
a cheap way is eppendorf and biorad.
Not affiliated with any company. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
- Posts: 11
- Joined: May 23 2012 3:13 pm
StepOnePlus from Life Technologies is a good buy. Easy to use and affordable. Recently, they have a sale.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
- PI of Posters
- Posts: 6467
- Joined: Jan 11 2006 12:40 pm
- Location: Mauna Kea
I agree with the StepOnePlus suggestion...they are still having a special through July. I have used the roche lightcycyler (old), biorad's earlier makes, and the StepOnePlus. It is easy to use and comes in the 48 or 96 well plate format (you can use strip tubes if you like though). The tower is pretty sturdy and straightforward. The software is easy to master as well. As far as reagents, I would shop around and not necessarily use AB's contracted Life tech supplies.
- Prolific Post-Master
- Posts: 214
- Joined: Nov 20 2003 6:26 pm
- Location: Cleveland, Ohio
Yep, StepOne is a good choice, although I've always found it weird that the VeriFlex Blocks (the independently controllable peltier blocks) are organized as columns instead of rows. It is indeed very sturdy and has an intuitive software.
BioRad's CFX96 Touch is also a great machine in my opinion, but it is a more expensive alternative. It's software is also very good and when you get the machine, you get a qBase license.
- Posts: 19
- Joined: Jan 22 2012 12:07 pm
Return to Real-Time qPCR/qRT-PCR Methods
Who is online
Users browsing this forum: No registered users and 1 guest