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PCR setup

Use this category for questions related to various quantitative PCR methods, including real time PCR, reverse transcription (RT)/PCR, qPCR, etc. For general PCR methods, continue to use the DNA/General PCR forum

Moderators: r.rosati, Suzanne

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PCR setup

Postby bms396 » Jul 05 2012 7:25 am

Hi,

Just wondering if anyone could give me some advice. I'm about to start doing some real time in a new lab I've just joined and they don't have a pCR hood in their own lab. They do however have a fume hood (with UV light). Would it be ok to give this a good clean down and use for PCR? Or am I best to 'borrow' a hood in another lab?

Any advice would be appreciated,

Many thanks!
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Re: PCR setup

Postby r.rosati » Jul 06 2012 7:32 pm

You want to absolutely, totally avoid air flow on your tubes. So a chemical hood is a big no-no. Good PCR hoods are those simple ones with an UV lamp and no air flow.
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Re: PCR setup

Postby bms396 » Jul 10 2012 7:41 am

OK thanks!

Advice much appreciated!
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