The height of amplification curve

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The height of amplification curve

Postby laleh » Jan 05 2017 12:40 am

Dear All
Hi
we are performing real time PCR for relative quantification and we have repeated the experiment with different master mix and CDNAs and new primer sets but every time our results show very low fluorescent height in amplification curve for our samples (below or around 1) and higher fluorescent for our NTC (around 2-3). The ct value for the test is around 22 and for the NTC around 32.
We have checked the product on the agarose gel and our test band are the region expected and we only have low amount of primer dimer in NTC.
Can anyone help me with this?
Thanks
laleh
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