Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.
Hi, I'm using mitochondrial JC-1 stain from "Sigma".
I did it few times and recently I get negative values after subtracting the blank from a sample value. Another question is concerning the wave length: I'm cheking the emision ratio 590nm/520nm and the curves not always show reciprocity. Can anyone help me?
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