Fluorescence Microscope

Use for discussion of various microscopy and imaging techniques, such as phase, fluorescence and confocal microscopy, electron microscopy, and various other imaging techniques.

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Fluorescence Microscope

Postby adopt » Jan 18 2013 3:40 am

Calling all lab personnel , I wanted to see if anyone is having the same frustration as myself.
I am not sure how much you use the fluorescence microscope but for us we usually need to do a quick check on transfection efficiency say GFP.
But in order to use the complex microscope in our lab it takes like 10 mins to start up the system, 5 mins to warm up the mercury burner, start up the PC switch the correct flurescence cubes and start to focus and observe. We are not doing any high end advanced microscopy work here mind you.
Do you guys and girls agree that if this could be simplified solution for pure "checking" of labeling it would save us lots of time?
Comments appreciated.
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Re: Fluorescence Microscope

Postby r.rosati » Jan 21 2013 8:53 am

If you're interested, there was a cool Benchmarks paper on Biotechniques some years ago, about using an LED source to visualize GFP fluorescence:

http://www.biotechniques.com/Biotechniq ... 45372.html

An LED light source wouldn't need the 5-minute startup time, nor you'd need to wait for it to be stable at running temperature before switching it off, or wait for it cool at room temperature again before switch it on again.
(You can actually skip that 5-minute waiting time after you switch on the burner, the lamp just won't be as bright. But don't switch it off before it's hot, or on again before it's cold, or the lamp might fail prematurely).
Best,

Roberto
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Re: Fluorescence Microscope

Postby Antikdarabok » Feb 08 2013 6:49 am

Can I offer you a better possibility?
You could see on the BLS Ltd. A company for specialized needs that we have a Fluorescent Attachment with which, you can work easy. Please take a look http://bls-ltd.com/FMA-04.html
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Re: Fluorescence Microscope

Postby ninja25 » Feb 20 2013 3:13 pm

Or you can do it in real-time with this inexpensive live cell microsope that operates in the cell incubator and does time-lapse imaging;
http://www.bulldog-bio.com/lumascope.html
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Re: Fluorescence Microscope

Postby brianrasnow » Feb 26 2013 8:37 pm

Bulldog is right -- Lumascope is super fast and easy to measure GFP expression or fluorophore distribution. It can sit on a desk or shelf, doesn't need to sit in an incubator. Leave it plugged into a usb port and its always ready to take pictures.
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Re: Fluorescence Microscope

Postby h2talal » Jun 27 2013 1:25 pm

Sounds like the perfect application for this: http://youtu.be/MzUan4DdCCY

3-color, LED illumination (quick on/off, no lamp warmup, etc). quick and easy.
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