Yes, that is what I meant, but I'm not really sure it's good advice, especially since I can't see what you are doing. As I mentioned I might be concerned of losing the genomic if it's still stuck to the proteins, although I think this might help. It is listed as an alternative in RNA guanidinium extractions. It may be safter to spin it out after the extraction, as Relaxin suggested.With regards to spinning out the solids, this would be performed between lysis and phenol:chloroform step? I would then retain supernatent and continue with the extraction.
What has happenned to me, on occasion, is when the lysate is very viscous while I pipet up the supernatant with my tip well away from the interphase, the tip grabs a piece of heavy, viscous "snot" that whips up the interphase at a distance from the tip, pulling solids up into the supernatant even though my tip was not near the interphase.The lysate looked extremely 'thick'/'gloopy' prior to addition of PC, are you suggesting that my DNA conc. is at such a level that it is pulling up (in want of a better description) the interphase proteins?
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