Finding specific cDNA's

[karel5_98]

What's the general procedure for finding out if a cDNA of a certain gene is available, and how should I order or ask for it?
I wonder if some database could give me the required information.

Thanks in advance.

[relaxin]

A number of companies sell expression-ready cDNA (or ORF) clones. For example, ATCC, Origene, and Addgene. If you want it for free, you may search PubMed with names of species and gene you want and see if someone else has cloned it. Then you can send them an e-mail and ask for it. Most scientists are nice enough to send it to you with the only restriction on research use only and that you will not pass it to a third party.

[Openbiotechnology]

its pretty easy and straight forward to pull whatever cDNA you want out of yourself assuming you want a human cDNA, or whatever else if you have the organism handy.

Use reverse transcriptase, do pcr, done.

[mchlbrmn]

its pretty easy and straight forward to pull whatever cDNA you want out of yourself assuming you want a human cDNA, or whatever else if you have the organism handy.

Use reverse transcriptase, do pcr, done.

You'd probably be violating safety rules if you do that. Human tissue makes it a BL2 procedure, and then there's consideration of 'informed consent' and 'research subject protection rules' about getting the tissue sample. It's a nightmare to consider all that dangerous biological material inside my body.
But, yeah, I agree it's not extremely difficult to clone genes yourself. The considerations are whether you have access to a tissue that expresses the gene(s), and the sequence should be verified by sequencing, so consider that if the gene is large this takes more effort.