Restriction enzyme problem

Use this category for questions directly related to DNA manipulation (isolation, purification, sequencing, etc.) and questions regarding general PCR methodologies

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Re: Restriction enzyme problem

Postby mchlbrmn » Jun 17 2009 3:45 pm

Good point that since this is a non-palindromic sequence, the recognition site on the complementary strand is a different sequence.
By "template strand" it seems you mean cDNA. I think this is a DNA template here "mtdna" (is that mitochondrial genome DNA?), so if it's DNA both strands will be present as template.
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Re: Restriction enzyme problem

Postby Onyourcase » Jun 17 2009 5:02 pm

It was a long time coming. Ok, you've given the sequence and your fragment lengths on digestion with mboII are 160bp, 86bp and 88bp. You are going to see only two bands on an agarose gel.
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