Standard protocol for DNA purification from Frozen Blood??

Use this category for questions directly related to DNA manipulation (isolation, purification, sequencing, etc.) and questions regarding general PCR methodologies

Moderators: r.rosati, mchlbrmn

Standard protocol for DNA purification from Frozen Blood??

Postby ashsu89 » Mar 04 2012 3:48 am

Does anyone have a reliable standard DNA purification protocol for extracting DNA from frozen blood samples? I have limited experience in bio techniques and have never done this type of procedures before. I was wondering if anyone have good suggestions of simple and reliable procedures I can follow without the use of phenol? I currently have frozen blood samples that are stored at around -80 degrees in Quiagene PreAnalytix Blood DNA tubes. However, since I could not purchase the kit, I need a standardized procedure as well as a list of reagents/buffers I need to help me purify the DNA from the blood samples.

Anyone have suggestions about what I should do?? I'm getting pretty helpless and desperate in trying to figure out a way... Thanks so much!!!
Posts: 1
Joined: Mar 04 2012 3:34 am

Re: Standard protocol for DNA purification from Frozen Blood

Postby biochembug » Mar 17 2012 8:49 am


What for you require the DNA? Regular PCR?

You may find several protocols which are based on proteinase K digestion followed by phenol extraction. For removal of proteins, you will have to do a phenol chloroform. I guess if you do not want column you will need a phenol extraction. I have also seen some papers showing direct PCR from blood samples. Google it or visit,,

Hope this helps!

Prolific Post-Master
Prolific Post-Master
Posts: 211
Joined: Aug 02 2006 9:02 pm
Location: USA

Return to DNA and General PCR Methods

Who is online

Users browsing this forum: No registered users and 1 guest