need recommendation for stable transduction of fibroblast ce

[memari]

Hello
I am going to do a stable transduction of fibroblast cells (SCC25). I search in Google and I found a lot of different products and ways which made me confused.
in this link says that I should not use fibroblast cells.
https://docs.google.com/viewer?a=v&q=cache:M_eRiCKOgzkJ:research.jax.org/faculty/mills/protocols/lentiviral_transfection.pdf+&hl=en&gl=ca&pid=bl&srcid=ADGEESgEtaBZ7OMm1ZHO-26bVuI3deQVEQqxTC38rrxJM0v4mop7fWsGwYpGc2KzKKeipa-1f0UHRNjyXUak3z1VC7Xj2CCBunI7oUMoLY50c0KKzbXI1_2tr5Oq35ZxxUqtcXsp_xmZ&sig=AHIEtbS3YrXqXR_vzo9kmRwMYUC_KXrOGw

SCC25 cells are very tough to transfect buy lipofectamine and similar products.
which product do you recommend?
which transduction is better for SCC25? baculovirus or lentivirus or ...?

-----
Babak

[CrowSan]

Hi - fibroblasts tend to be pretty tough cells (one of the first cells into the wound environment whilst neutrophil oxygen bursts are going off) so they can take a lot of punishment. If I were you I would first try Ca PO4 precipitation (I used this on Rat-2 fibroblasts a long time ago with ~ 20-30% transductrion).
You can make up the components yourself so it is very cheap. There are a number of protocols on-line with ingredients etc. this link is just to the first one http://www.flemingtonlab.com/Protocols/ ... Transf.pdf
CrSn