Solid-State DNA Quantification

Use this category for questions directly related to DNA manipulation (isolation, purification, sequencing, etc.) and questions regarding general PCR methodologies

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Solid-State DNA Quantification

Postby Thelongwindingcode » Feb 06 2018 11:01 am

I would like some creative suggestions on how you might go about quantifying the absolute amount of nucleotides hybridised to a primer perminantly tethered to a surface, such as a slide etc. :D
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Re: Solid-State DNA Quantification

Postby mdfenko » Feb 06 2018 6:48 pm

you can tag the nucleotides with fluorescent or radioactive labels
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Re: Solid-State DNA Quantification

Postby Thelongwindingcode » Feb 15 2018 5:39 am

Thank you for your response!
This is what I was thinking, do you know if cy3 and cy5 are FRET pairs? The literature seems to suggest they are, but people are still using them to quantify numbers of molecules in the same sample.
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Re: Solid-State DNA Quantification

Postby mdfenko » Feb 15 2018 9:13 am

that's the pair i used when 2-color hybridizing microarrays.
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Re: Solid-State DNA Quantification

Postby Thelongwindingcode » Feb 15 2018 12:52 pm

Excellent! Thank you!
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