ssRNA annealing

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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ssRNA annealing

Postby nekameneka » Jul 05 2017 5:04 am

Hi guys,

I have another question about annealing RNA to get dsRNA. In my previous experience annealing ssRNA which will produce dsRNA bigger then 1kbp I used gel shift to check my annealing where dsRNA travels a little bit slower then it's ssRNA before annealing. So now I have question does the same rule apply for smaller RNAs?? Because I generated 50bp RNA and my "dsRNA" is traveling little bit faster then ss, what happened here?
Thanks!
nekameneka
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