UPDATE: Yup, you can split one large tube into a bzillion smaller tubes using a sterile stainless spatula, approximately 100ul per tube. I also found that when I use Trireagent it gets a little sloppy if I spin it at 4C, but if I spin it in the centrifuge in the cold room (which I would guess is closer to 6-8C (?), it makes a nice gel phase without sticking to the sides. Also, when I am using it for PCI cleanups or just a second BCP/chloroform cleanup, I never get the "smears" on the side like I occasionally do with the Trireagent. FWIW. One box of large size tubes could potentially last you, like, forever. Silicone grease seems to work ok too, no real difference in the side-by-side except that it seems to smear a little worse with Trireagent. All in all, for shaky-handed folks like me this stuff is a godsend! I'll never extract RNA without them (and Pellet Paint) again.