mchlbrmn wrote:The protocols I am familiar with use, I believe,
for DNA: 3/5M KAc, pH 5.2 (3M K, and 5M Acetate), or
for RNA: 2M Kac, pH 4.2
Perhaps there an error or misunderstanding in your protocol, and you are trying to do something impossible.
If you are performing the guanidinium isothiocyanate RNA preparation, the solution must have the lower pH KAc because only under acid conditions will the RNA stay in the aqueous and the DNA go into the organic phase during the extraction. I think the pH is probably more important than the exact molarity.
Also, for some solutions it is impossible to dissolve the salt completely until you also adjust the pH at the same time.
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