Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)
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Hi there. I have acquired several very old cell samples (protists, primarily), stored at -20C for 5 to 10 years or more. I was wondering whether it would be viable to try an mRNA extraction, or that's just too long in storage. They were collected for the purpose of small molecule extraction, so there's a pretty significant mass, and as far as I can tell they were simply centrifuged down and stored wet without any treatment. Thanks for any help!
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-20 C is just not cold enough to keep RNA intact.
You can use it for protein and DNA extraction.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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