today I´ve run a normal non-denaturating 1% agarose gel with RNA (~180ng) isolated from jurkat cells for quality and integrity assessment.
normally the 28srRNA band should have twice the intensity then the 18srRNA band, but this not the case for me....its even the other way round. Furthermore the two RNA-molecules appear to be too small of size. 28sRNA is approximately 1,5 kb big and 18 srRNA around 500bp. This is usually a sign of RNA-degradation, right? I really dont know why this should be the case because i worked very cleanly and thouroughfully. i even denaturated my RNA at 70°C for 1 minute before applying the RNA onto the gel.
For me there are two explanations:
1) i eluted in nuclease-free water (ambion)
2) jurkat cells are tumor cells and those have in general degraded rRNA
help and thoughts are appreciated.
Users browsing this forum: No registered users and 1 guest