Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)
Moderators: mchlbrmn, Abhijeet Bakre
I am trying to transfect a dsRNA fragment (one strand attached to a quencher, one strand attached to a fluorophore) into Hek293 cells using RNAimax but the transfection efficiency has been really low. Does anybody have any idea about an optimal concentration of RNAimax or RNA or number of cells per well?
I will be really grateful for any suggestions!
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