Thanks for your answers, I see that I should have been more precisely with my second reference, where I was referring to the Procedure described within the "Bacterial cultures and extraction of total tRNA"-section.
"Extraction of cellular RNA was done following a reported procedure (Ehrenstein, G. (1965) Methods Enzymol
., 12, 588-595) with minor modifications. Cells were resuspended in 1.5 vol of 1.0 Tris-HCl, 10 mM MgCl2, pH 7.2. Phenol (1.5 vol) saturated with the same solution was added and, after 1 h mixing at 4°C, the phases were separated by centrifugation. The aqueous phase was recovered and the nucleic acids precipitated with ethanol."
My question was more about the reason, why the extraction system used by Chomczynski & Sacchi contained guanidine thiocyanate, phenol and chloroform and why that from Cayana et al. in contrast contained only phenol.
Anyway, meanwhile I have performed further literature research and must say that it has never been so hard to find proper informations about a topic. On the one hand this was because of the fact, that to the time when the essential work on nucleic acid preparations or isolations was done, one used to say “soluble RNA” or “sRNA” and not “tRNA”, but which I used as central element within my search phrases. On the other hand, I encountered the problem that most of the articles about RNA extraction were describing a procedure for the use on non-bacterial (mostly mammalian) cells, mostly following the aim of mRNA extraction to perform transcriptional analysis, and could not be simply applied on E. coli cells with the aim to isolate tRNA for the use within a coupled in-vitro transcription/translation. But, while reading through the 21 papers I found about the topic, the whole question cleared up step by step and finally the puzzle was completed as follows:
Within the topic of nucleic acid isolation, one has to differ between further subtopics, respectively focusing on isolation of:
- Total DNA and RNA
- Total RNA without DNA, containing Insoluble, large-size RNA molecules and soluble, small ones (rRNA, mRNA)
- Soluble, small-size RNA molecules without large ones (tRNA)
- aminoacylated tRNAs
- Unmodified tRNA
To begin with the final result of my literature research: soluble, small-size RNA molecules / tRNA (sRNA) can be extracted directly from bacterial cells without the need of cell lysis just by the use of phenol as single extraction agent. This relies upon the action of phenol on cell membrane permeability, making it possible for small particles, like tRNA, to pass through, while mRNA, rRNA are already too big. If the interest lies upon the isolation of total RNA, containing ribosomal or mRNA, then acetone becomes integrated into the phenol extraction procedure for the reasons of altering membrane permeability to a point where the large-size RNA molecules can pass outside the cell.
(http://www.ncbi.nlm.nih.gov/pmc/article ... 2-0130.pdf
The sole use of phenol is limited to bacterial (or maybe yeast as well) cells. For extraction of nucleic acid from mammalian cells, it was necessary to develop procedures like the guanidine thiocyanate/phenol/chloroform-procedure due to the need of reagents for cell lysis, nuclease deactivation and antioxidatives.