RNA stability in Tissues in RNAlater

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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RNA stability in Tissues in RNAlater

Postby DinaRoxenTool » Apr 11 2015 1:45 pm

According to the QIAGEN protocol, the RNA in the tissues is stable in RNAlater for a period of 4 weeks if stored at 4 degrees. What can happen if the samples were stored 3 more days after the "deadline" and then extracted?
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Re: RNA stability in Tissues in RNAlater

Postby r.rosati » Apr 11 2015 6:01 pm

Nothing much - you're slightly past the date where the company guarantees that RNAlater will keep the RNA intact, but if you used the correct amount of solution, three days won't likely make any difference.
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Re: RNA stability in Tissues in RNAlater

Postby mobio7 » Jun 09 2016 2:39 am

It should work just fine :)
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