Is this RNA degraded?

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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Is this RNA degraded?

Postby zwang022 » Nov 17 2015 1:09 am

[Please See Attachment] I extracted total RNA from a PNT2 cell line and ran on an agarose gel. The two major rRNA bands appear in the 1-2kb regions but look blurry and fuzzy. Is this a sign of degradation, simply overloaded, or is it ready for downstream experiments qRT-PCR? Shown on the left side is a 2-log ladder standard.
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Re: Is this RNA degraded?

Postby mdfenko » Nov 17 2015 7:58 am

is that a formaldehyde (denaturing) gel?

is the ladder dna or rna?
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Re: Is this RNA degraded?

Postby r.rosati » Nov 17 2015 8:17 am

Also: did you heat your RNA before loading? Was it in pure water, i.e. no buffer whatsoever?
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