promega RNA dilution buffer

Use this category for the exchange of ideas, methodologies and references regarding the isolation, manipulation and analysis of RNA. (Extraction protocols, Northern Blot analysis, RNase Protection, Differential Display, In Vitro Transcription, etc.)

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promega RNA dilution buffer

Postby Esraa Bassam » Jan 05 2016 2:04 am

i am using promega RNA extraction kit which contain RNA dilution buffer (RDA) and give good results
but by using Qiagene Protocol the yield and purity affected and also downstream processes
Does anyone know why or what are the contents of this buffer ?
Esraa Bassam
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Re: promega RNA dilution buffer

Postby relaxin » Jan 05 2016 11:44 am

Reagents and buffers from different kits (especially from different companies) should not be mixed. Most of the reagents and buffers are proprietary. You can just use RNase-free water to dilute the RNA.
Retired academic researcher. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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