how to decide RNA RNA interaction

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how to decide RNA RNA interaction

Postby hasmat buchad » Jan 22 2017 9:49 am

need help

I am doing RNA-RNA interaction using gel mobility shift assay. i hv two RNAs RNA_1 and RNA_2
RNA_1 is labeled with dig and incubated with unlabeled RNA_2 to chek binding or interaction.
On blot interaction will be detected by shift in mobility of labeled. in my case I get a shift in labeled RNA_1. shift is observed same place where secondary structure of RNA_2 is present. Don't know how to conclude...

here I attached an image where blot is shown along with EtBr staining to check the position of RNA_2 and its Secondary structure.
plz plz help with this
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hasmat buchad
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Joined: Dec 17 2016 2:28 am

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