HiTrap Heparin HP for purification of DNA binding proteins.

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HiTrap Heparin HP for purification of DNA binding proteins.

Postby memari » Jun 27 2012 10:05 am

I have bought "HiTrap Heparin HP" for purification of DNA binding proteins.

http://www.gelifesciences.com/webapp/wc ... 2/17040601

This the first time that I am going to do such a purification and I do not have any knowledge and experiences,

Do you have a protocol for using this colums for purification of DNA binding proteins?

As I am going to use these purified proteins for next steps such as DNA-Affinity Chromatography,

what should I use as Elution buffer?

My concern is NaCl in Elution buffer of "HiTrap Heparin HP" for dissociating the proteins from Heparin, because it interferes with DNA-Affinity Chromatography. I use NaCl in my elution buffer of DNA-Affinity Chromatography to dissociate proteins from bounded Oligos on the beads.

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Re: HiTrap Heparin HP for purification of DNA binding protei

Postby mdfenko » Jun 27 2012 10:53 am

talent does what it can
genius does what it must
i do what i get paid to do
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Re: HiTrap Heparin HP for purification of DNA binding protei

Postby leekaming » Jul 05 2012 8:54 am

NaCl is usually used in elution from Heparin column.
You can dialyse to remove NaCl after elution from heparin column.
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