Lysis buffer

Use this category for questions regarding problems manipulating proteins in molecular biology applications (expression, detection, etc.)

Moderators: mdfenko, leekaming

Lysis buffer

Postby newbie17425 » Nov 16 2016 2:36 pm

My proteins of interest are inside the cell membrane. Is there any specific lysis buffer or can I use RIPA buffer to lyse my tissues. My tissue of interest is the hippocampus.

Prolific Post-Master
Prolific Post-Master
Posts: 137
Joined: May 22 2015 3:23 pm

Re: Lysis buffer

Postby relaxin » Nov 17 2016 12:03 pm

For Western blot, RIPA plus protease inhibitor cocktail will be fine.
Retired academic researcher. Mention of a specific product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
PI of Posters
PI of Posters
Posts: 7187
Joined: Jan 11 2006 12:40 pm
Location: Mauna Kea

Return to Protein Methods

Who is online

Users browsing this forum: No registered users and 1 guest