Cloning of repetitive sequence of DNA

[kedardm]

I wish to clone a repetitive sequence of DNA into a plasmid vector. What mutation in the E.coli genome would ensure successful maintenance of this DNA sequence in E.Coli?

[relaxin]

Try use E. coli strain such as "SURE" for cloning difficult sequences.

[s_atif_ali2002]

I wish to clone a repetitive sequence of DNA into a plasmid vector. What mutation in the E.coli genome would ensure successful maintenance of this DNA sequence in E.Coli?

racA1, a deletion in this gene would allow an E.coli to maintain direct repetitive sequences. You can use SURE, I routinely use Stbl-2 cells from Invitrogen.