ifhmn wrote: The question is, why for certain genes, thay use - strand and others use + strand. If I want to look up the promoter sequence (- strand) should I look at the upstream of downstream sequence?
God may be able to answer your first question.
As for the second question, the promoter sequence is likely to be upstream from the Exon 1, but can be embedded within the Exon 1 sequence.
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