I am doing flow for the first time and need someone to tell me if the way I am analysing my results is fine:
I have a test antibody (CD34) and an isotype control. I have run each in a different tube but on the same cell line.
Now, in the results, I had to gate that particular population of course and have got mean flouresence values, which are higher for isotype controls.
Do I substract the mean of test from the isotype control mean? What's the best way to analyse flow results?
Help much appreciated.