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I am trying to convert protein readings from ug/ml to ug/well. I had 200ul of RIPA buffer in a well, collected in, then ran 2.5ul of that sample in an BCA protein assay (I diluted the my samples 1:10, so 2.5 sample in 22.5ul diluent). Then let's say after measuring it and calculating I get ~6000ug/ml. How do I convert that measurement to ug/well? Do I just multiply that concentration by .2ml to get the original amount in the well?
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Yes, if the 6000 ug/ml has been corrected by dilution factor of 10 (i.e. 600 ug/ml [from BCA assay] x 10 [dilution factor].
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