Antibody concentration and conjugation kit

[gertruckis]

Hello, I'm having problems trying to solve a concentration problem. I want to conjugate an antibody with biotin, and in the kit it's said that the recomended amount of antibody is 200 µg with a recomended volume of 40-100 µl with a concetration of 1-4mg/ml. The antibody information says that it has a concentration of 100 µg at 1mg/ml, and i dont understand if I have 100µg in 1ml or what, and how i can calculate the volume i need to take for 200µg of antibody. I thought about searching the molar weight but i dont find anything, and sincerelly I didn't much chemistry in few years and i dont remember much, so I'm a little bit lost now.

Thank you!!

[relaxin]

Your antibody is 100 ug IgG total, and the concentration is 1 mg/ml or 1 ug/ul. In other words, you have only 100 ug of antibody in 100 ul. There is no way to get 200 ug antibody for conjugation.