Non-professional visitors (i.e., lay people, high school and undergraduate students) or professionals from other fields should use this forum for general questions regarding molecular biology. No guarantee that they'll be answered but you can always try!
Moderators: r.rosati, mchlbrmn
Hello, I'm having problems trying to solve a concentration problem. I want to conjugate an antibody with biotin, and in the kit it's said that the recomended amount of antibody is 200 µg with a recomended volume of 40-100 µl with a concetration of 1-4mg/ml. The antibody information says that it has a concentration of 100 µg at 1mg/ml, and i dont understand if I have 100µg in 1ml or what, and how i can calculate the volume i need to take for 200µg of antibody. I thought about searching the molar weight but i dont find anything, and sincerelly I didn't much chemistry in few years and i dont remember much, so I'm a little bit lost now.
- Posts: 1
- Joined: Jul 23 2012 5:42 am
- Location: Tarragona, Spain
Your antibody is 100 ug IgG total, and the concentration is 1 mg/ml or 1 ug/ul. In other words, you have only 100 ug of antibody in 100 ul. There is no way to get 200 ug antibody for conjugation.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
- PI of Posters
- Posts: 6469
- Joined: Jan 11 2006 12:40 pm
- Location: Mauna Kea
Return to Student Questions
Who is online
Users browsing this forum: No registered users and 1 guest