IRES-sequence, something similar?

[Antibes]

Hi, I've got a question and I was hoping someone could help me. For my research proposal (undergraduate student) I'm suggesting a variety of contructs for transfection. I would like to combine my protein of interest with a truncated NGFR, to be able to select my transfected cells with an antibody. So I would just use a construct with an IRES in between, that's fine. However, I have two proteins of interest and I would also like to make a combined construct with the two proteins and the NGFR: proteinA_IRES_proteinB_IRES_NGFR, but I guess I can't use the IRES twice in the same constuct? My superviser said something about a similar sequence for in between my two proteins, something called N2 or N2a. But I can't find anything about it! Does anyone know what he would be talking about?

[relaxin]

You may find your answer in the following paper:

de Felipe P. Polycistronic viral vectors. Curr Gene Ther. 2002 Sep;2(3):355-78.

[Antibes]

Thanks!! That is exactly what I was looking for, but it was very difficult to find if you don't know exactly what you're looking for.. You might just have saved my honours certificate.. Thank you so much!!