I need list of PCR inhibitors

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I need list of PCR inhibitors

Postby Noha » Dec 28 2006 9:02 pm

Hi

I'm working in Molecular Biology diagnostic lab and I need to know what are the most common pcr inhibitors, but I can't find any useful link or papre.

could any one help me, plz?
Noha
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Postby Noha » Jan 03 2007 7:48 pm

pleeeeeeeeeeeeeeeeeeeeeeeeeeeeez help me I need an answer :roll:
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Postby chason » Jan 08 2007 2:01 pm

There are a lot of things that can inhibit PCR
You might find the Alkami PCR Guide helpful, but I don't think they have a comprehensive list:
http://www.gene-quantification.de/quick-guide-pcr.pdf

Some common things that can inhibit PCR are excess EDTA, NaCl, SDS or other detergent, and any phenol or ethanol. There's also stuff that can precipitate with your DNA during extraction (like proteins and polysaccharides) that will inhibit your PCR. People also have problems with inhibition from DNA samples from feces, blood, soil, and formalin fixed samples, among others.

Do you have a specific problem with inhibition, or were you looking for general information?
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Postby Noha » Jan 08 2007 3:16 pm

THANK YOU SOOOOOOOO MUCH

I was looking for general information about exteraction from plasma samples :D
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