recombinant E.Coli

[Gayathri Selvaraju]

hi,
why intracellular enzyme is preferable if we used recombinant e.coli (harboring gene for protease production) for protease production? is there possible for extracellular to have higher protease activity compared intracellular?

[relaxin]

In general, the protein sequence of the recombinant protease will determine whether it will be expressed intracellularly or extracellularly. However, over-express mammalian protease in E. coli often results in insoluble inclusion bodies. The protease needs to be refolded before it can be active. Extracellular protease is often secreted into the medium in the active form.