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EtBr 5μl of a 10mg/ml stock solution per 50 ml of agarose

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EtBr 5μl of a 10mg/ml stock solution per 50 ml of agarose

Postby gene26 » Jul 01 2012 5:18 pm

What will be the final concentration.. if EtBr is added as 5μl of a 10mg/ml per 50 ml of agarose

how to calculate...:(
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Re: EtBr 5μl of a 10mg/ml stock solution per 50 ml of agaros

Postby mchlbrmn » Jul 02 2012 11:00 am

gene26 wrote:What will be the final concentration.. if EtBr is added as 5μl of a 10mg/ml per 50 ml of agarose

how to calculate...:(
Do a simple ratio:
5ul / 50,000 ul x 10mg/ml =

(or, 1ul/ml x 10mg/ml = 10ug/ml (1/1000).
5ul makes it 50 ug/ml.
/50ml makes it 1 ug/ml.)
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Re: EtBr 5μl of a 10mg/ml stock solution per 50 ml of agaros

Postby gene26 » Jul 03 2012 1:56 am

Yeps Thank u...
its like C1V1=C2V2...:)
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Re: EtBr 5μl of a 10mg/ml stock solution per 50 ml of agaros

Postby relaxin » Jul 03 2012 1:59 pm

Yup, this is the only formula you have to remember in dealing with volume and concentration. It works with percentage, molarity, and g/L, etc.
Not affiliated with any company. Mention of a specifc product does not imply my endorsement of the product. No conflict of interest or guarantee to work on the advice given. Do as I say, not as I do. Not liable to the loss of your valuable samples.
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