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When I tested the Wolbachia of Tribolium confusum adults using STE, I got nothing. But it can works for pupae.When I tested the other beetle(Larsioderma serricorne) using STE, it can works for adults and pupae. Using columns for extraction, pupae and adults of both beetle species test positive. I have obtained this result several times.I will appraciate if anyone can tell me the probable reason or maybe someone have the same problem with my. I am look foward to get the answer.
Thanks for your help!
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