Joining overlapping sequences together

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Joining overlapping sequences together

Postby fmorton » Mar 23 2017 10:55 am

Hi, I have amplified 3 regions of the hepatitis c virus genome. I used MEGA to align them and formed a contig. I'm trying to right a straight forward and clear method on how I did this but I'm struggling. I'm dyslexic so I can't phrase what I want to say easily.

At the moment I have something on the lines of this : once aligned a contig can be generated by reassembling region 1,2 and 3 to ensure the sequences do not overlap.

I don't think this is very scientific so any other way of phrasing this would help me out a lot.
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Re: Joining overlapping sequences together

Postby relaxin » Mar 23 2017 11:53 am

Just say: A contiguous sequence is generated by aligning the three overlapping DNA sequences.
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