witch_doctor wrote:Exactly so.
Methanol makes the pores on the membrane 'open' so as to facilitate protein binding. When using PVDF, you can decrease methanol in your transfer buffer, or omit it altogether, provided of course you have activated your membrane first. This allows greater binding efficiency than nitrocellulose.
RRam wrote:To add in more, you can store the membrane after protein transfer simply by drying and covering with the filter paper. But before proceeding to further step like blocking, you should wet the membrane with methanol, followed by the water/buffer (to replace the methanol) which you would for the sucessive step.
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